Journal: Scientific Reports

Article Title: Examining potential confounding factors in gene expression analysis of human saliva and identifying potential housekeeping genes

doi: 10.1038/s41598-022-05670-5

Figure Lengend Snippet: The graph represents raw Ct values (threshold cycles) of potential housekeeping genes (n = 10) analyzed for the 40 samples from 10 donors (four time points per donor), arranged in ascending order of raw unamplified Ct values. For each gene, the Ct values measured via qRT-PCR with cDNA without pre-amplification as well as after 14× pre-amplification is shown. Symbols represent geometric mean values and error bars reflect the standard error of mean per gene. Vertical and horizontal grey dashed lines show the cut-off (mean Ct ≤ 30). Three of the genes ( ACTB , ATP6 and B2M , highlighted in grey area) showed un-amplified Ct-values < 30 indicating that no pre-amplification for adequate detection will be required.

Article Snippet: In the present work, 10 different TaqMan® Gene Expression Assays ( ACTB , Hs01060665_g1; B2M , Hs00187842_m1; GUSB , Hs00939627_m1; MT-ATP6 , Hs02596862_g1; PGK1 , Hs00943178_g1; PP1A , Hs99999904_m1; RPL13A , Hs04194366_g1; RPLP0 , Hs02992885_s1; TBP , Hs00427620_m1; YWHAZ , Hs01122445_g1) were utilized and pooled to enable the multiplex amplification of specific cDNA targets.

Techniques: Quantitative RT-PCR, Amplification

Journal: Scientific Reports

Article Title: Examining potential confounding factors in gene expression analysis of human saliva and identifying potential housekeeping genes

doi: 10.1038/s41598-022-05670-5

Figure Lengend Snippet: Raw Ct values (threshold cycles) of three candidate house-keeping genes ( ACTB , ATP6 and B2M ) as well as a combination of them (arithmetic mean) are depicted over time (four time point each: 9 am—0 h; 3 pm—6 h; 9 pm—12 h; 9 am next day—24 h) for each donor. They were fulfilling the criteria for being an appropriate housekeeping gene in this context.

Article Snippet: In the present work, 10 different TaqMan® Gene Expression Assays ( ACTB , Hs01060665_g1; B2M , Hs00187842_m1; GUSB , Hs00939627_m1; MT-ATP6 , Hs02596862_g1; PGK1 , Hs00943178_g1; PP1A , Hs99999904_m1; RPL13A , Hs04194366_g1; RPLP0 , Hs02992885_s1; TBP , Hs00427620_m1; YWHAZ , Hs01122445_g1) were utilized and pooled to enable the multiplex amplification of specific cDNA targets.

Techniques:

Journal: Scientific Reports

Article Title: Examining potential confounding factors in gene expression analysis of human saliva and identifying potential housekeeping genes

doi: 10.1038/s41598-022-05670-5

Figure Lengend Snippet: Overview of the housekeeping gene expression results (raw Ct values either without pre-amplification or after 14× pre-amplification) and the significant correlations with sociodemographic and epidemiological characteristics.

Article Snippet: In the present work, 10 different TaqMan® Gene Expression Assays ( ACTB , Hs01060665_g1; B2M , Hs00187842_m1; GUSB , Hs00939627_m1; MT-ATP6 , Hs02596862_g1; PGK1 , Hs00943178_g1; PP1A , Hs99999904_m1; RPL13A , Hs04194366_g1; RPLP0 , Hs02992885_s1; TBP , Hs00427620_m1; YWHAZ , Hs01122445_g1) were utilized and pooled to enable the multiplex amplification of specific cDNA targets.

Techniques: Gene Expression

Journal: PLoS Pathogens

Article Title: Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells

doi: 10.1371/journal.ppat.1005660

Figure Lengend Snippet: A) Heatmap of expression values of differentially expressed genes (DEGs) in human macrophages not infected (mock) or infected with WA314 or WA314ΔYopM . Duplicates (two different donors) of primary human macrophages were infected without (mock) or with Yersinia enterocolitica WA314 or WA314ΔYopM for 6 h. Total RNA was prepared from each sample and subjected to RNA-seq. The scaled expression of each set of replicates, denoted as the row Z-score, is plotted in a red-blue color scale. Red indicates high and blue indicates low expression. Only genes with an absolute log2-fold change greater or equal 2 and p-value smaller or equal 0.01 are shown. These genes were hierarchically clustered (complete linkage) according to their expression profiles. The resulting distinct sets of genes are indicated by the orange-, purple-, blue- and yellow color bar. The number of DEGs in each cluster is indicated. B) Analysis of IL-10 expression in Yersinia infected human macrophages from different donors . Total RNA was isolated from primary human macrophages that were mock infected or infected with WA314 or WA314ΔYopM for 6 h. The RNA was subjected to quantitative RT-PCR using human IL-10 specific primers. IL-10 expression was normalized to expression of three housekeeping genes (GAPDH, TBP, B2M). For each condition triplicate samples of macrophages derived from seven different donors (Donor_1 to Donor_7) were investigated (data from Donor_4 to Donor_7 in ). Each bar in graph represents mean ± SD of values from all 7 donors; **p<0.01, *p<0.05. C) Induction of IL-10 expression requires interaction of YopM with RSK . Experimental procedures as in B) with the difference that macrophages were infected with WA314ΔYopM(pYopM_1–481) and WA314ΔYopM(pYopM). Each Bar in graph represents mean ± SD of values from 3 different donors; **p<0.01. D) DDX3 knockdown increases IL-10 expression in Yersinia infected human macrophages . Primary human macrophages were transfected with control siRNA (mock, siCtrl) or DDX3 siRNA (siDDX3 No. 3) for 72 h and not infected (mock) or infected with WA314 for 6 h. Total RNA was subjected to quantitative RT-PCR as in B). Each Bar in graph represents mean ± SD of values from 4 different donors; *p<0.05, ***p<0.001. Western blot verifies DDX3 knockdown in the macrophages.

Article Snippet: As reference the TagMan Gene Expression Assay for GAPDH (Hs02758991_g1), TATA-box binding protein (TBP) (Hs00427620_m1) and beta-2-microglobulin (B2M) (Hs00187842_m1) was used (all from Thermo Fisher Scientific Waltham, Massachusetts, USA).

Techniques: Expressing, Infection, RNA Sequencing, Isolation, Quantitative RT-PCR, Derivative Assay, Knockdown, Transfection, Control, Western Blot

Journal: Nature communications

Article Title: Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12.

doi: 10.1038/s41467-025-59930-9

Figure Lengend Snippet: Fig. 7 | CDK12-controlled DNA repair is a vulnerability in NUP98::KDM5A-driven leukemia. A Cell viability assay of primary human NUP98-rearranged AML cells, healthy donor BM MNC and CD34+ progenitors treated with indicated con- centrations of THZ531 for 3 days (n = 3 technical replicates). B GI50 values from cell viability assays of murine AML cells treated with THZ531 for 3 days. C RT-qPCR analysis of dTAG-NUP98::KDM5A cells treated with THZ531 (2 µM, 24 h) showing log2FC values (n = 3 technical replicates). D Gene set enrichment analysis of RNA- seq data from a doxycycline-controlled NUP98::KDM5A cell line (Tet-Off) after 5 days of doxycycline-induced NUP98::KDM5A downregulation compared to DMSO. E Time series plot of Tet-Off RNA-seq data showing gene expression levels of DNA double strand break repair genes at day 3 and day 5 after NUP98::KDM5A downregulation normalized to NUP98::KDM5A-expressing cells, highlighting selected genes (left), with a density plot of normalized expression of all genes

Article Snippet: For the generation of the CDK12 wild type overexpression construct pCR8/GW_TOPO_Cdk12_Nterm_FlagHa (Addgene plasmid #127177) expressing the mouse Cdk12 transgene (NM_001109626.1 isoform) was cloned into pLX303-Gw (Addgene plasmid #25897) using Gateway LR Clonase II EnzymeMix (Invitrogen, USA) according the manufacturer’s protocol.

Techniques: Viability Assay, Quantitative RT-PCR, RNA Sequencing, Gene Expression, Expressing

Journal: Redox Biology

Article Title: Specific delivery of delta-5-desaturase siRNA via RNA nanoparticles supplemented with dihomo-γ-linolenic acid for colon cancer suppression

doi: 10.1016/j.redox.2018.101085

Figure Lengend Snippet: Construction of tumor-targeting 3WJ nanoparticle. (A) Illustration for the design of a functional 3WJ RNA nanoparticle. (B) Dynamic light scattering characterization showing the hydrodynamic size of RNA nanoparticles. (C) Atomic force microscopy image of extended 3WJ-nanoparticles. (D) Cell-binding test via flow cytometry in HCA-7/C29 cells after being incubated with 100 nM 3WJ-Alexa 647 or 3WJ-EpCAM-Alexa 647 (in 100 µL PBS). Cells incubated with PBS only (without nanoparticles) served as controls. (E) Confocal microscopy analysis of 3WJ-EpCAM-Alexa 647 particles entering HCA-7/C29 cells. Cell skeletons were stained with Alexa 488 and cell nuclei with DAPI. (F) qRT-PCR analysis for D5D gene knockdown efficiency in HCA-7/C29 cells after treatment with 10 nM of 3WJ nanoparticles with different compositions. Data represent mean ± SD with three separate experiments. *: significant difference with p < 0.05.

Article Snippet: The apparent hydrodynamic sizes of the assembled RNA nanoparticles (40 μM in 1 ×Tris buffer) were measured by a Zetasizer nano-ZS (Malvern Instrument, LTD) at 25 °C.

Techniques: Functional Assay, Microscopy, Binding Assay, Flow Cytometry, Incubation, Confocal Microscopy, Staining, Quantitative RT-PCR, Knockdown

Journal: Redox Biology

Article Title: Specific delivery of delta-5-desaturase siRNA via RNA nanoparticles supplemented with dihomo-γ-linolenic acid for colon cancer suppression

doi: 10.1016/j.redox.2018.101085

Figure Lengend Snippet: 3WJ nanoparticles and DGLA supplementation suppress xenograft tumor growth. (A) In vivo biodistribution analysis of 3WJ-EpCAM-Alexa 647 particles in organs and tumor tissues from mice bearing HCA-7 tumors at 8-h post-injection. (B) Ultrasound images of tumor growth in mice before and after 4-week treatments. Note, week 0 indicates the starting point of treatment, which is two weeks after cancer cell implantation. (C) Relative tumor volume measured twice a week using a digital caliper during the treatment. Data represent mean±SD with six tumor samples. *: significant difference with p < 0.05. Insert: photos of tumor tissues at the end of treatment.

Article Snippet: The apparent hydrodynamic sizes of the assembled RNA nanoparticles (40 μM in 1 ×Tris buffer) were measured by a Zetasizer nano-ZS (Malvern Instrument, LTD) at 25 °C.

Techniques: In Vivo, Injection

Journal: Redox Biology

Article Title: Specific delivery of delta-5-desaturase siRNA via RNA nanoparticles supplemented with dihomo-γ-linolenic acid for colon cancer suppression

doi: 10.1016/j.redox.2018.101085

Figure Lengend Snippet: 3WJ nanoparticles and DGLA supplementation promote 8-HOA formation in xenograft tumors. (A) Immunofluorescence analysis of D5D expression levels in tumor tissues. (B) LC/MS quantification of DGLA levels from tumor tissues. (C) LC/MS quantification of AA levels from tumor tissues. (D) DGLA/AA ratio from tumor tissues. (E) GC/MS quantification of 8-HOA from tumor tissues. All the quantification data represent mean±SD with six tumor samples. *: significant difference with p < 0.05.

Article Snippet: The apparent hydrodynamic sizes of the assembled RNA nanoparticles (40 μM in 1 ×Tris buffer) were measured by a Zetasizer nano-ZS (Malvern Instrument, LTD) at 25 °C.

Techniques: Immunofluorescence, Expressing, Liquid Chromatography with Mass Spectroscopy, Gas Chromatography-Mass Spectrometry

Journal: Biochemical and biophysical research communications

Article Title: The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD + decline.

doi: 10.1016/j.bbrc.2019.03.199

Figure Lengend Snippet: (A-B) Relative mRNA levels of senescence-related genes in HUVECs treated with (A) X-ray irradiation (IR) (n=7–9) or (B) γ-IR (n=4–8). (C) Relative mRNA levels of CD38 in HUVECs treated with X-ray (n=7–9) or -IR (n=6–7). (D) Relative mRNA levels of senescence-related genes in irradiated MEFs (n=6). Relative mRNA levels (A-D) were determined by qRT-PCR. (E) CD38 relative enzymatic activity in multiple independent irradiation-induced senescent MEFs (passage 5–9) (n=2–3). Data are mean ± SE, *p < 0.05 vs control.

Article Snippet: The expression changes were calculated relative to control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID Cd138 Mm01220906_m1 Cdkn2a Mm0049449_m1 Cdknla Mm04205640_g1 Il1b Mm00434228_m1 Il6 Mm00446190_m1 Nos2 Mm00440502_m1 Hprt Mm01545399_m1 Tbp Mm00446971_m1 Open in a separate window TaqMan Gene Expression Assays (Mouse) table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID CD38 Hs01120071_m1 CDKN1A Hs00355782_m1 CDKN2A Hs00923894_m1 CDKN2D Hs00176481_m1 CXCL8 Hs00174103_m1 GAPDH Hs02758991_g1 IL6 Hs00985639_m1 TP53 Hs01034249 Open in a separate window TaqMan Gene Expression Assays (Human)

Techniques: Irradiation, Quantitative RT-PCR, Activity Assay, Control

Journal: Biochemical and biophysical research communications

Article Title: The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD + decline.

doi: 10.1016/j.bbrc.2019.03.199

Figure Lengend Snippet: (A) mRNA expression of selected SASP factors (IL-6, IL-8, MCP-1) in senescent HUVECs, determined by qRT-PCR (n=7–9). (B) CD38 activity (n=3–5) and (C) a representative immunoblot showing CD38 levels in BMDMs treated with various factors found in the SASP. Data are mean ± SE, *p < 0.05 vs control.

Article Snippet: The expression changes were calculated relative to control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID Cd138 Mm01220906_m1 Cdkn2a Mm0049449_m1 Cdknla Mm04205640_g1 Il1b Mm00434228_m1 Il6 Mm00446190_m1 Nos2 Mm00440502_m1 Hprt Mm01545399_m1 Tbp Mm00446971_m1 Open in a separate window TaqMan Gene Expression Assays (Mouse) table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID CD38 Hs01120071_m1 CDKN1A Hs00355782_m1 CDKN2A Hs00923894_m1 CDKN2D Hs00176481_m1 CXCL8 Hs00174103_m1 GAPDH Hs02758991_g1 IL6 Hs00985639_m1 TP53 Hs01034249 Open in a separate window TaqMan Gene Expression Assays (Human)

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Western Blot, Control

Journal: Biochemical and biophysical research communications

Article Title: The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD + decline.

doi: 10.1016/j.bbrc.2019.03.199

Figure Lengend Snippet: (A) Scheme showing production of control conditioned media (CM) and SASP (senescent cell conditioned media) from HUVECs or PDPs for treatment of non-senescent cells. (B) Relative CD38 mRNA expression and activity in BMDMs treated with CM or SASP from x-ray- (n=7–9) or γ-IR-treated (n=3) HUVECs. (C and D) CD38 mRNA expression and activity in BMDMs treated with CM or SASP from PDP originating from (patient 1 in C) or (patient 2 in D) (n=2–3). (E) Relative CD38 expression (n=4–6) in HUVECs treated with CM or SASP from γ-IR MEFs. Data are mean ± SE, *p < 0.05 vs control.

Article Snippet: The expression changes were calculated relative to control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID Cd138 Mm01220906_m1 Cdkn2a Mm0049449_m1 Cdknla Mm04205640_g1 Il1b Mm00434228_m1 Il6 Mm00446190_m1 Nos2 Mm00440502_m1 Hprt Mm01545399_m1 Tbp Mm00446971_m1 Open in a separate window TaqMan Gene Expression Assays (Mouse) table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID CD38 Hs01120071_m1 CDKN1A Hs00355782_m1 CDKN2A Hs00923894_m1 CDKN2D Hs00176481_m1 CXCL8 Hs00174103_m1 GAPDH Hs02758991_g1 IL6 Hs00985639_m1 TP53 Hs01034249 Open in a separate window TaqMan Gene Expression Assays (Human)

Techniques: Control, Expressing, Activity Assay

Journal: Biochemical and biophysical research communications

Article Title: The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD + decline.

doi: 10.1016/j.bbrc.2019.03.199

Figure Lengend Snippet: (A) Representative immunoblots demonstrating levels of CD38 in BMDMs treated with CM (control conditional media) or SASP (x-ray- or γ-IR-induced senescent cell conditioned media) from PDPs or HUVECs. (B) mRNA expression of other M1 markers, namely IL-6, IL-8, and Nos2 (inducible nitric oxide synthase), in BMDMs treated with CM or SASP from PDPs. (C) Model of SASP-induced CD38 expression in macrophages. Data are mean ± SE, *p < 0.05 vs control.

Article Snippet: The expression changes were calculated relative to control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID Cd138 Mm01220906_m1 Cdkn2a Mm0049449_m1 Cdknla Mm04205640_g1 Il1b Mm00434228_m1 Il6 Mm00446190_m1 Nos2 Mm00440502_m1 Hprt Mm01545399_m1 Tbp Mm00446971_m1 Open in a separate window TaqMan Gene Expression Assays (Mouse) table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID CD38 Hs01120071_m1 CDKN1A Hs00355782_m1 CDKN2A Hs00923894_m1 CDKN2D Hs00176481_m1 CXCL8 Hs00174103_m1 GAPDH Hs02758991_g1 IL6 Hs00985639_m1 TP53 Hs01034249 Open in a separate window TaqMan Gene Expression Assays (Human)

Techniques: Western Blot, Control, Expressing

Journal: Biochemical and biophysical research communications

Article Title: The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD + decline.

doi: 10.1016/j.bbrc.2019.03.199

Figure Lengend Snippet: TaqMan Gene Expression Assays (Human)

Article Snippet: The expression changes were calculated relative to control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID Cd138 Mm01220906_m1 Cdkn2a Mm0049449_m1 Cdknla Mm04205640_g1 Il1b Mm00434228_m1 Il6 Mm00446190_m1 Nos2 Mm00440502_m1 Hprt Mm01545399_m1 Tbp Mm00446971_m1 Open in a separate window TaqMan Gene Expression Assays (Mouse) table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Probe ID CD38 Hs01120071_m1 CDKN1A Hs00355782_m1 CDKN2A Hs00923894_m1 CDKN2D Hs00176481_m1 CXCL8 Hs00174103_m1 GAPDH Hs02758991_g1 IL6 Hs00985639_m1 TP53 Hs01034249 Open in a separate window TaqMan Gene Expression Assays (Human)

Techniques: Gene Expression

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A The RNA-seq data were obtained from the TGCA-GBM cohort. B The microarray gene expression data were taken from the GSE108474 cohort. C GBM tissues obtained from Evangelisches Krankenhaus in Oldenburg were analyzed by qRT-PCR. All data show lower expression of RD3 in GBM compared to non-tumor samples. The statistical analysis used an unpaired t test. The statistical analysis results are shown in supp. Table and ( P value: * < 0.05, ** < 0.01, **** < 0.0001).

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: RNA Sequencing, Microarray, Gene Expression, Quantitative RT-PCR, Expressing

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A , B Overall survival-probability analysis according to RD3 transcript level. C – E Diagnostic test of RD3 in GBM in different cohorts as indicated (AUC area under curve, 95% CI 95% confidence interval).

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: Diagnostic Assay

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A Overview over RD3 structure as electrostatic surface potential representation, with residues highlighted by dashed circles in physiological and patient-derived conditions from two different perspectives. B Zoom onto individual residues in native (upper row) and patient-derived conditions (lower row) in RD3. The native residues and patient-derived mutations analyzed in this study are shown by their side-chain moiety and an overlay of the transparent electrostatic surface potential with secondary structure representation. C Western blot test of RD3 and its variants in HEK293T cell transfection. The monoclonal RFP antibody (1:2000) was used to detect the inserted RFP tag, the band around 27 kDa represents the RFP, and at 49 kDa represents the fusion protein of RD3 and RFP; the mouse β-Tubulin antibody (1:2000) was used as a housekeeping protein with a molecular mass around 55 kDa. For the original full-length blot membrane, see supp. Fig. .

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: Derivative Assay, Western Blot, Transfection, Membrane

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A RD3 wild-type compared to control group. B RD3 wild type compared to variants. The statistical analysis used two-way ANOVA, and results are shown in supp. Tables , ( P value: *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001).

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: Control

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A HEK293T cells transfected with the RD3 variants. B HEK293T cells transfected with empty vector and Mock control. C . The summary cell cycle distribution of 5 replicates. The ANOVA test was performed to analyze the percentage of cells across cell cycle D G1 phase, E S phase, and F G2/M phase. Statistical analysis used two-way ANOVA, and results are shown in supp. Tables – ( P value: *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001).

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: Transfection, Plasmid Preparation, Control

Journal: Cell Death Discovery

Article Title: Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis

doi: 10.1038/s41420-025-02475-z

Figure Lengend Snippet: A RD3 and its variants transfected HEK293T cells. B Vector and Mock control. C Summary of cell apoptotic analysis 24 h after transfection. Apoptotic analysis of cells in D . RD3 and control group, E RD3 and its variants. The statistical analysis used two-way ANOVA, and results are shown in supp. Tables , ( P value: *< 0.05, **< 0.01, ***< 0.001, ****< 0.0001).

Article Snippet: The human RD3 probe, and two housekeeping TBP and HPRT1 probes were purchased from Thermo Fisher Scientific (RD3: Hs01650935_m1, TBP: Hs00427620_m1, and HPRT1: Hs01003270_g1).

Techniques: Transfection, Plasmid Preparation, Control